19/30404344 DC - BS EN ISO 14501. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

19/30404344 DC

BS EN ISO 14501. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

Status : Current, Draft for public comment   Published : December 2019

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This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 μg/kg for milk powder and LOD is 0,005 μg/kg for liquid milk. The limit of quantification(LOQ) is 0,1 μg/kg for milk powder and LOQ is 0,01 μg/kg for liquid milk.

The method is also applicable to low fat milk, skimmed milk, low fat milk powder and skimmed milk powder.

CAUTION 1

The method described in this protocol requires the use of solutions of aflatoxin M1. Aflatoxins are carcinogenic to humans. Attention is drawn to the statement made by the International Agency for Research on Cancer (WHO)[1,2].

CAUTION 2

Protect the laboratory in which the analyses are performed adequately from daylight and keep aflatoxin standard solutions protected from light, e.g. by using aluminium foil.

CAUTION 3

The use of non-acid-washed glassware (e.g. tubes, vials, flasks, beakers, syringes) for aqueous aflatoxin solutions may cause loss of aflatoxin.
Moreover, brand new laboratory glassware, before coming into contact with aqueous solutions of aflatoxin, should be soaked in dilute acid (e.g. sulfuric acid, 2 mol/l) for several hours, then rinsed well with distilled water to remove all traces of acid (check to ensure pH is in the range 6 to 8).

CAUTION 4

Use decontamination procedures for laboratory wastes such as solid compounds, solutions in organic solvents, aequeous solutions and spills, and for glassware coming into contact with carcinogenic materials. Suitable decontamination procedures have been developed and validated by the International Agency for Research on Cancer (WHO)[1,2].




Standard Number19/30404344 DC
TitleBS EN ISO 14501. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography
StatusCurrent, Draft for public comment
Publication Date18 December 2019
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)ISO 5725-1:1994, ISO 5725-2:1994, ISO 707
International RelationshipsISO/DIS 14501
Draft Expiry Date04 February 2020
DescriptorsDetermination of content, Dried milk, Aflatoxin, Specimen preparation, Milk, Affinity chromatography, Dairy products, Chemical analysis and testing, Test equipment, Test specimens, Immunoassay, Liquid chromatography, Food products, Food testing
ICS67.100.10
Title in FrenchLait et lait en poudre — Détermination de la teneur en aflatoxine M1 — Purification par chromatographie d'immunoaffinité et détermination par chromatographie en phase liquide à haute performance
CommitteeAW/5
PublisherBSI
FormatA4
DeliveryYes
Pages19
File Size877 KB
NotesWarning: this draft is not current beyond its expiry date for comments.
Price£20.00


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