19/30396561 DC - BS EN 17425. Foodstuffs. Determination of ergot alkaloids in cereals and cereal products by dSPE clean-up and LC-MS/MS

19/30396561 DC

BS EN 17425. Foodstuffs. Determination of ergot alkaloids in cereals and cereal products by dSPE clean-up and LC-MS/MS

Status : Current, Draft for public comment   Published : August 2019

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This document describes a method for the determination of the sum total of six ergot alkaloids (ergocornine, ergometrine, ergocristine, ergotamine, ergosine and ergocryptine) and their -inine epimer pairs by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) after clean-up by dispersive solid phase extraction (SPE).

The method has been validated for cereals and cereal-based food products.

The method has been validated in the range 13,2 µg/kg to 168 µg/kg for the sum of the twelve ergot alkaloids, in rye flour, rye bread and cereal products (breakfast cereal, infant breakfast cereal, and crispbread) that contained rye as an ingredient, as well as seeded wholemeal flour and a barley and rye flour mixture.

Method performance was satisfactory in the range 24,1 µg/kg to 168 µg/kg, however at lower concentrations RSDR values were greater than 44 %, and HorRat values exceeded 2,0, indicating the method may not be fully suitable at concentrations below 24 µg/kg for sum of ergot alkaloids, although it is suitable for screening at these concentrations. Method performance may be improved by inclusion of an isotopically labelled internal standard, but this was not available at the time of the method validation study.




Standard Number19/30396561 DC
TitleBS EN 17425. Foodstuffs. Determination of ergot alkaloids in cereals and cereal products by dSPE clean-up and LC-MS/MS
StatusCurrent, Draft for public comment
Publication Date06 August 2019
Normative References(Required to achieve compliance to this standard)EN ISO 3696
Informative References(Provided for Information)EN ISO/IEC 17025, ISO 5725 (all parts)
International RelationshipsprEN 17425
Draft Expiry Date24 September 2019
DescriptorsMeasuring instruments, Measurement characteristics, Measurement, Density measurement, Food crops, Test equipment, Test specimens, Calibration, Crops, Bulk density, Agricultural products, Cereals
ICS67.050
67.060
Title in GermanLebensmittel - Bestimmung von Ergotalkaloiden in Getreiden und Getreideerzeugnissen mit dSPE-Reinigung und LC-MS/MS
CommitteeAW/275
PublisherBSI
FormatA4
DeliveryYes
Pages46
File Size592 KB
NotesWarning: this draft is not current beyond its expiry date for comments.
Price£20.00


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