ASTM E3143 - 18b - Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

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ASTM E3143 - 18b

Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

Status : Current   Published : November 2018



1.1 This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4  This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard NumberASTM E3143 - 18b
TitleStandard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes
Publication Date15 November 2018
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors cryo-TEM, liposomes, vitrificiation
File Size492 KB

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