ASTM E3180 - 18 - Standard Test Method for Quantification of a Bacillus subtilis Biofilm Comprised of Vegetative Cells and Spores Grown Using the Colony Biofilm Model

ASTM E3180 - 18

Standard Test Method for Quantification of a Bacillus subtilis Biofilm Comprised of Vegetative Cells and Spores Grown Using the Colony Biofilm Model

Status : Current   Published : October 2018

Format
PDF

Format
HARDCOPY



1.1 This test method specifies the operational parameters required to grow and quantify a Bacillus subtilis biofilm comprised of vegetative cells and endospores (spores) using the colony biofilm method (CBM). 2,3 The resulting biofilm is representative of static environments that can develop a sporulating biofilm rather than being representative of one particular environment.

1.2 This test method utilizes a modified CBM to grow the biofilm. The CBM uses a semipermeable membrane on an agar plate as the biofilm growth surface and nutrient source. 2,3 In this test method, membranes are inoculated and incubated for a total of 8 days to promote sporulation within the biofilm.

1.3 This test method describes how to sample and analyze the biofilm for vegetative cells and spores. Biofilm population is expressed as total colony forming units (CFU) and spores per membrane.

1.4 Basic microbiology training is required to perform this test method.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7  This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.




Standard NumberASTM E3180 - 18
TitleStandard Test Method for Quantification of a Bacillus subtilis Biofilm Comprised of Vegetative Cells and Spores Grown Using the Colony Biofilm Model
StatusCurrent
Publication Date01 October 2018
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors biofilm, Bacillus subtilis, colony, membrane, sampling, spore
ICS07.100.01
75.160.20
PublisherASTM
FormatA4
DeliveryYes
Pages4
File Size78 KB
Price£36.00


 Your basket
Your basket is empty

Multi-user access to over 3,500 medical device standards, regulations, expert commentaries and other documents


BSOL

The faster, easier way to work with standards


Worldwide Standards
We can source any standard from anywhere in the world


Tracked Changes

Understand the changes made to a standard with our new Tracked Changes version