19/30389588 DC - BS EN ISO 11063. Soil quality. Direct extraction of soil DNA

19/30389588 DC

BS EN ISO 11063. Soil quality. Direct extraction of soil DNA

Status : Current, Draft for public comment   Published : September 2019

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The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.

The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.




Standard Number19/30389588 DC
TitleBS EN ISO 11063. Soil quality. Direct extraction of soil DNA
StatusCurrent, Draft for public comment
Publication Date10 September 2019
Normative References(Required to achieve compliance to this standard)ISO 18400-206
Informative References(Provided for Information)No other standards are informatively referenced
International RelationshipsISO/DIS 11063
Draft Expiry Date27 October 2019
DescriptorsQuality, Information exchange, Data sampling, Soils
ICS13.080.30
Title in FrenchQualité du sol — Extraction directe de l'ADN du sol
CommitteeEH/4
PublisherBSI
FormatA4
DeliveryYes
Pages17
File Size326 KB
NotesWarning: this draft is not current beyond its expiry date for comments.
Price£20.00


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