PD CEN/TS 17303:2019 Foodstuffs. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments

PD CEN/TS 17303:2019

Foodstuffs. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments

Status : Current   Published : April 2019

Format
PDF

Format
HARDCOPY



What is this standard about?

This document describes a procedure for the identification of single fish and fish fillets to the level of genus or species.

The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) [1] or the cytochrome c oxidase I gene (cox1, syn COI) [2], [3] or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases [4], [5]. The methodology allows the identification of a large number of commercially important fish species.

The decision whether the cytb or cox1 gene segment or both are used for fish identification depends on the declared fish species, the applicability of the PCR method for the fish species and the availability of comparative sequences in the public databases.

This method has been successfully validated on raw fish fillets, however, laboratory experience is available that it can also be applied to processed, e.g. cold smoked, hot smoked, salted, frozen, cooked, fried, deep-fried samples.

This document is usually unsuitable for the analysis of highly processed foods, e.g. tins of fish, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex fish products containing mixtures of two or more fish species.




Standard NumberPD CEN/TS 17303:2019
TitleFoodstuffs. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments
StatusCurrent
Publication Date24 April 2019
Normative References(Required to achieve compliance to this standard)EN ISO 24276, ISO 16577, ISO 24276
Informative References(Provided for Information)EN ISO 21571:2005, ISO 21571:2005, ISO 20813, ISO/IEC 17025, IEC 17025
International RelationshipsCEN/TS 17303:2019
DescriptorsMussels, Toxins, Chemical analysis and testing, Liquid chromatography, Fish (meat), Food testing, Determination of content, Food products, Shellfish
ICS67.120.30
Title in FrenchProduits alimentaires. Codes-barres d'ADN de poissons et de produits à base de poissons à l'aide de segments de gènes mitochondriaux du cytochrome b et cyctochrome c oxydase I
Title in GermanLebensmittel. DNA-Barcoding von Fisch und Fischprodukten anhand definierter mitochondrialer Cytochrom-b- und Cytochrom-c-Oxidase-I- Genabschnitte
CommitteeAW/275
ISBN978 0 539 00390 1
PublisherBSI
FormatA4
DeliveryYes
Pages28
File Size738 KB
Price£182.00


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