BS EN 17280:2019 - Foodstuffs. Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS/MS

BS EN 17280:2019

Foodstuffs. Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS/MS

Status : Current   Published : October 2019

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This document specifies a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2 and T-2) and zearalenone (ZEN) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after clean-up by solid phase extraction (SPE).

The method has been validated with samples of wheat, wheat flour, and wheat crackers. The wheat and the wheat flour were prepared from a mixture of wheat and fungi infected wheat kernels. The wheat crackers were baked from wheat flour and water spiked with the target mycotoxins.

Validation levels for NIV ranged from 27,7 ?g/kg to 378 ?g/kg.

Validation levels for DON ranged from 234 ?g/kg to 2420 ?g/kg.

Validation levels for 3-acetyl-DON ranged from 18,5 ?g/kg to 137 ?g/kg.

Validation levels for 15-acetyl-DON ranged from 11,4 ?g/kg to 142 ?g/kg.

Validation levels for HT-2 ranged from 6,6 ?g/kg to 134 ?g/kg.

Validation levels for T-2 ranged from 2,1 ?g/kg to 37,6 ?g/kg.

Validation levels for ZEN ranged from 31,6 ?g/kg to 230 ?g/kg.

Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5], however, this has not been validated in a collaborative study.




Standard NumberBS EN 17280:2019
TitleFoodstuffs. Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS/MS
StatusCurrent
Publication Date17 October 2019
Normative References(Required to achieve compliance to this standard)ISO 3696, EN ISO 3696
Informative References(Provided for Information)ISO 5725 (all parts), ISO/IEC 17025, EN ISO/IEC 17025
International RelationshipsEN 17280:2019
Draft Superseded By18/30375154 DC
DescriptorsMass spectrometry, High performance liquid chromatography, Cereal products, Cereals, Zearalenone, Food
ICS67.060
Title in FrenchProduits alimentaires. Dosage de la zéaralénone et des trichothécènes y compris du déoxynivalénol (DON) et ses dérivés acétylés (3-acétyl-DON et 15-acétyl-DON), du nivalénol (NIV) et des toxines T-2 et HT-2 dans les céréales et les produits céréaliers par CL-SM/SM
Title in GermanLebensmittel. Bestimmung von Zearalenon und Trichothecenen einschließlich Deoxynivalenol und den acetylierten Derivaten (3-Acetyl-Deoxynivalenol und 15-Acetyl-Deoxynivalenol, Nivalenol sowie T-2- und HT-2-Toxin in Getreide und Getreideerzeugnissen mit LC-MS/MS
CommitteeAW/275
ISBN978 0 539 00386 4
PublisherBSI
FormatA4
DeliveryYes
Pages52
File Size1.025 MB
Price£254.00


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