BS EN 15634-2:2019 - Foodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR

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BS EN 15634-2:2019

Foodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR

Status : Current   Published : October 2019

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This document specifies a method for the detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).

Real-time PCR (polymerase chain reaction) detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082 1) of celery (Apium graveolens).

The method has been validated on emulsion-type sausages (Bavarian “Leberkäse”) spiked with celery. For this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 °C for 60 min [1].

This document is intended to be used in addition to EN 15842 and EN 15634?1.




Standard NumberBS EN 15634-2:2019
TitleFoodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR
StatusCurrent
Publication Date11 October 2019
Cross ReferencesEN 15634-1:2019, BS EN 15634-1:2019, EN 15842, EN ISO/IEC 17025, ISO/IEC 17025
ReplacesPD CEN/TS 15634-2:2012
International RelationshipsEN 15634-2:2019
Draft Superseded By18/30371926 DC
DescriptorsBiochemical methods of analysis, Agricultural products, Food testing, Allergies, Qualitative analysis
ICS07.100.30
67.120.10
Title in FrenchProduits alimentaires. Détection des allergènes alimentaires par des méthodes d'analyse de biologie moléculaire Céleri (Apium graveolens). Détection d'une séquence d'ADN spécifique dans des saucisses cuites par PCR en temps réel
Title in GermanLebensmittel. Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren Sellerie (Apium graveolens). Nachweis einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time-PCR
CommitteeAW/275
ISBN978 0 580 99646 7
PublisherBSI
FormatA4
DeliveryYes
Pages24
File Size1.379 MB
Price£176.00


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