BS EN ISO 21268-3:2019 - Soil quality. Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil-like materials. Up-flow percolation test

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BS EN ISO 21268-3:2019

Soil quality. Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil-like materials. Up-flow percolation test

Status : Current   Published : October 2019



This document specifies a test, which is aimed at determining the leaching behaviour of inorganic and organic substances from a soil and soil-like materials. The method is a once-through up-flow percolation test under standardized conditions of flow rate. The material is leached under dynamic hydraulic conditions. The document has been developed to measure the release of inorganic and organic substances from soil and soil-like material as well as to produce eluates for subsequent ecotoxicological testing. For ecotoxicological testing, see ISO 15799[6] and ISO 17616[7]. The test results enable the distinction between different release patterns, for instance wash-out and release under the influence of interaction with the matrix, when approaching local equilibrium between material and leachant.

This test method produces eluates, which can subsequently be characterized by physical, chemical and ecotoxicological methods in accordance with existing standard methods. The results of eluate analysis are presented as a function of the liquid/solid (L/S) ratio. The test is not suitable for substances that are volatile under ambient conditions.


Volatile organic substances include the low-molecular-weight substances in mixtures such as mineral oil.


It is not always possible to optimize test conditions simultaneously for inorganic and organic substances and optimum test conditions can also vary between different groups of organic substances. Test requirements for organic substances are generally more stringent than those for inorganic substances. The test conditions suitable for measuring the release of organic substances will generally also be applicable to inorganic substances.


Within the category of organic substances, a significant difference in behaviour exists between the more polar, relatively water-soluble compounds and apolar, hydrophobic organic substances (HOCs). In the latter case, mechanisms of release (e.g. particle-bound or dissolved organic carbon-bound) can be more crucial as well as sorption losses of soluble HOCs on different materials with which they come in contact (e.g. bottles, filters). The test and the results should be used for leaching of organic substances only with thorough consideration of the specific properties of the substances in question and the associated potential problems.


For ecotoxicological testing, eluates representing the release of both inorganic and organic substances are needed. In this document, ecotoxicological testing is also meant to include genotoxicological testing.


The test is generally not suitable for soils with hydraulic conductivities below 10 â??8 m/s (see also Annex B). It can be difficult to maintain the designated flow rate already in the range of saturated hydraulic conductivity between 10 â??7 m/s and 10 â??8 m/s.

The application of this test method alone is not sufficient for the determination of the leaching behaviour of a material under specified conditions different to those from the test procedure, since this generally requires the application of several test methods, behavioural modelling and model validation. This document does not address issues related to health and safety. It only determines the leaching properties as outlined in Clause 4.

Standard NumberBS EN ISO 21268-3:2019
TitleSoil quality. Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil-like materials. Up-flow percolation test
Publication Date30 October 2019
Cross ReferencesISO 10523, ISO 3696, ISO 7027-1, ISO 11465, ISO 5725-1:1994, ISO 5667-3:2018, BS ISO 5725-2:2019, ISO 5725-2:2019, ISO 18400-101, ISO 18400-202, ISO 11464, ISO 18400-105, ISO 18400-104, ISO 15799, DIN 19528, EN 12920, ISO 23909, ISO 17616, ISO 18772, CEN/TS 14405:2004
International RelationshipsEN ISO 21268-3:2019,ISO 21268-3:2019
Draft Superseded By18/30364136 DC
DescriptorsLeaching, Soils, Chemical analysis and testing, Quality, Testing
Title in FrenchQualité du sol. Modes opératoires de lixiviation en vue d'essais chimiques et écotoxicologiques ultérieurs des sols et matériaux du sol Essai de percolation à écoulement ascendant
Title in GermanBodenbeschaffenheit. Elutionsverfahren für die anschließende chemische und ökotoxikologische Untersuchung von Boden und von Bodenmaterialien Perkolationstest im Aufwärtsstrom
ISBN978 0 580 99416 6
File Size1.868 MB

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