ASTM E2562 - 17 - Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow using CDC Biofilm Reactor

ASTM E2562 - 17

Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow using CDC Biofilm Reactor

Status : Current   Published : April 2017

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PDF

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HARDCOPY



1.1 This test method specifies the operational parameters required to grow a reproducible ( 1)2Pseudomonas aeruginosa ATCC 700888 biofilm under high shear. The resulting biofilm is representative of generalized situations where biofilm exists under high shear rather than being representative of one particular environment.

1.2 This test method uses the Centers for Disease Control and Prevention (CDC) Biofilm Reactor. The CDC Biofilm Reactor is a continuously stirred tank reactor (CSTR) with high wall shear. Although it was originally designed to model a potable water system for the evaluation of Legionella pneumophila( 2), the reactor is versatile and may also be used for growing and/or characterizing biofilm of varying species ( 3-5).

1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log 10 colony forming units per surface area.

1.4 Basic microbiology training is required to perform this test method.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.7  This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.




Standard NumberASTM E2562 - 17
TitleStandard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow using CDC Biofilm Reactor
StatusCurrent
Publication Date01 April 2017
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors Biofilm|, Coupon, Growth reactor, <emph type="ital">Pseudomonas aeruginosa</emph>, Reactor, Sampling shear
ICS07.100.01
PublisherASTM
FormatA4
DeliveryYes
Pages6
File Size161 KB
Price£40.00


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