ASTM E2196 - 17 - Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor

ASTM E2196 - 17

Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor

Status : Current   Published : April 2017

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1.1 This test method is used for growing a reproducible ( 1)2Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method describes how to sample and analyze biofilm for viable cells.

1.2 Although this test method was created to mimic conditions within a toilet bowl, it can be adapted for the growth and characterization of varying species of biofilm (rotating disk reactor—repeatability and relevance ( 2)).

1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log 10 colony forming units per surface area (rotating disk reactor—efficacy test method ( 3)).

1.4 Basic microbiology training is required to perform this test method.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.7  This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.




Standard NumberASTM E2196 - 17
TitleStandard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
StatusCurrent
Publication Date01 April 2017
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors Analysis, Biofilm, Coupon, Growth reactor, <emph type="ital">Pseudomonas aeruginosa</emph>, Reactor, Sampling
ICS07.100.01
PublisherASTM
FormatA4
DeliveryYes
Pages6
File Size132 KB
Price£40.00


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