BS EN ISO 9167:2019 - Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography

BS EN ISO 9167:2019

Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography

Status : Current   Published : July 2019

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This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.

This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.

NOTE

This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.

Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.

The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.




Standard NumberBS EN ISO 9167:2019
TitleRapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography
StatusCurrent
Publication Date01 July 2019
Normative References(Required to achieve compliance to this standard)ISO 3696, ISO 5502, ISO 665, ISO 771, ISO 664
Informative References(Provided for Information)ISO 5725:1986, ISO 5725 (all parts), ISO 5500, ISO 21294
ReplacesBS EN ISO 9167-1:1995+A1:2013, BS 4289-9:1993
International RelationshipsEN ISO 9167:2019,ISO 9167:2019
Draft Superseded By17/30361509 DC
DescriptorsReproducibility, Vegetable oils, Plant products, Specimen preparation, Extraction methods of analysis, Measurement characteristics, Chemical analysis and testing, Cruciferae, Liquid chromatography, Column chromatography, Test specimens, Chromatography, Rape seed oil, Control samples, Quantitative analysis, Test equipment, Determination of content, Testing conditions
ICS67.200.20
Title in FrenchGraines et tourteaux de colza. Dosage des glucosinolates. Méthode par chromatographie liquide à haute performance
Title in GermanRapssamen und Rapsschrot. Bestimmung des Glucosinolatgehaltes. Verfahren mittels Hochleistungsflüssigchromatographie
CommitteeAW/307
ISBN978 0 580 98758 8
PublisherBSI
FormatA4
DeliveryYes
Pages38
File Size1.154 MB
Price£214.00


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