20/30360293 DC - BS EN 17521. Foodstuffs. Determination of Alternaria toxins in tomato, wheat and sunflower seeds by SPE clean-up and HPLC-MS/MS

20/30360293 DC

BS EN 17521. Foodstuffs. Determination of Alternaria toxins in tomato, wheat and sunflower seeds by SPE clean-up and HPLC-MS/MS

Status : Current, Draft for public comment   Published : May 2020

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This document specifies a procedure for the determination of five Alternaria toxins in wheat, tomato puree and sunflower seeds by high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS).

The method has been validated with both naturally contaminated and spiked samples of wheat, tomato puree and sunflower seeds.

Validation levels for altenuene (ALT) ranged from 2,18 μg/kg to 13,8 μg/kg.

Validation levels for alternariol (AOH) ranged from 1,82 μg/kg to 46,7 μg/kg.

Validation levels for alternariol monomethyl ether (AME) ranged from 1,29 μg/kg to 47,2 μg/kg.

Validation levels for tentoxin (TEN) ranged from 5,29 μg/kg to 218 μg/kg.

Validation levels for tenuazonic acid (TEA) ranged from 41,8 μg/kg to 1 618 μg/kg.

Limits of quantification of 1 μg/kg for ALT (except in wheat and sunflower seeds), AOH and AME; 5 μg/kg for TEN and 10 μg/kg for TEA or lower are achievable using this method.




Standard Number20/30360293 DC
TitleBS EN 17521. Foodstuffs. Determination of Alternaria toxins in tomato, wheat and sunflower seeds by SPE clean-up and HPLC-MS/MS
StatusCurrent, Draft for public comment
Publication Date28 May 2020
Normative References(Required to achieve compliance to this standard)EN ISO 3696, ISO 3696
Informative References(Provided for Information)ISO 5725 (all parts), EN ISO/IEC 17025
International RelationshipsprEN 17521
Draft Expiry Date20 July 2020
DescriptorsTest equipment, Proteins, Food testing, Performance, Genetically modified organisms, Sampling methods, Food products, Contaminant determination (food), Quantitative analysis
ICS67.050
Title in FrenchProduits alimentaires - Détermination de la teneur en toxines d'Alternaria dans la tomate, le blé et les graines de tournesol par chromatographie liquide couplée à la spectrométrie de masse en tandem
Title in GermanLebensmittel - Bestimmung von Alternariatoxinen in Tomaten, Weizen und Sonnenblumenkernen mit Flüssigchromatographie und Tandem-Massenspektrometrie
CommitteeAW/275
PublisherBSI
FormatA4
DeliveryYes
Pages39
File Size569 KB
NotesWarning: this draft is not current beyond its expiry date for comments.
Price£20.00


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