BS EN ISO 16649-3:2015 - Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide

BS EN ISO 16649-3:2015

Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide

Status : Current   Published : June 2015

Format
PDF

Format
HARDCOPY



This part of ISO 16649 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli , by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following:

  • products intended for human consumption and the feeding of animals;

  • environmental samples in the area of food production and food handling.

The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.

A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause 11.

This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.




Standard NumberBS EN ISO 16649-3:2015
TitleMicrobiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
StatusCurrent
Publication Date30 June 2015
Normative References(Required to achieve compliance to this standard)ISO 6887-4, ISO 6887-6, ISO 6887-5, ISO 6887-1, ISO 6887-3, ISO 7218, ISO 11133, ISO 6887-2
Informative References(Provided for Information)ISO 7251
ReplacesDD ISO/TS 16649-3:2005
Amended ByCorrigendum, February 2017
DescriptorsBiological analysis and testing, Culture techniques, Food fit for human consumption, Food testing, Animal feed, Food products, Microbiological analysis, Microbiology, Escherichia coli, Coliform bacteria, Test specimens, Count methods (microbiology), Bacteria count methods
ICS07.100.30
Title in FrenchMicrobiologie de la chaîne alimentaire. Méthode horizontale pour le dénombrement des Escherichia coli bêta-glucuronidase positive. Recherche et technique du nombre le plus probable utilisant le bromo-5- chloro-4-indolyl-3 ß-D-glucuronate
Title in GermanMikrobiologie der Lebensmittelkette. Horizontales Verfahren zur Zählung von ß-Glucuronidase-positiven Escherichia coli. Nachweis und Bestimmung der wahrscheinlichsten Keimzahl unter Verwendung von 5- Brom-4-Chlor-3-Indol-ß-D-Glucuronid
CommitteeAW/9
ISBN978 0 580 97525 7
PublisherBSI
FormatA4
DeliveryYes
Pages22
File Size2.133 MB
Price£130.00


 Your basket
Your basket is empty

Multi-user access to over 3,500 medical device standards, regulations, expert commentaries and other documents


Worldwide Standards
We can source any standard from anywhere in the world


BSOL

The faster, easier way to work with standards


Tracked Changes

Understand the changes made to a standard with our new Tracked Changes version