ASTM E2694 - 16 - Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

ASTM E2694 - 16

Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

Status : Current   Published : October 2016

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1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

1.3 This test method is equally suitable for use in the laboratory or field.

1.4 The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL.

1.5 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.




Standard NumberASTM E2694 - 16
TitleStandard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
StatusCurrent
Publication Date01 October 2016
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors Adenosine triphosphate, ATP, Bacteria, Bioburden, Biodeterioration, Biomass, Fungi, Metalworking, Microbial contamination, Microbiology, Microorganisms
ICS75.100
PublisherASTM
FormatA4
DeliveryYes
Pages11
File Size188 KB
Price£45.00


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