BS EN ISO 12966-1:2014 - Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Guidelines on modern gas chromatography of fatty acid methyl esters

BS EN ISO 12966-1:2014

Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Guidelines on modern gas chromatography of fatty acid methyl esters

Status : Current   Published : January 2015

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This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs).

The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids of the triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMEs), which are determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation.

The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols, to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards, FAMEs from C4 to C26 can be determined, including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.

For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoid interferences with the solvent peak and in order to reduce differences in detector responses.




Standard NumberBS EN ISO 12966-1:2014
TitleAnimal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Guidelines on modern gas chromatography of fatty acid methyl esters
StatusCurrent
Publication Date31 January 2015
Normative References(Required to achieve compliance to this standard)ISO 12966-2, ISO 12966-3, ISO 12966-4
Informative References(Provided for Information)No other standards are informatively referenced
ReplacesBS EN ISO 15304:2002, BS EN ISO 5508:1995, BS 684-2.35:1990
International RelationshipsEN ISO 12966-1:2014,ISO 12966-1:2014
Amended ByCorrigendum, June 2015.
Draft Superseded By13/30281563 DC
DescriptorsFats, Animal fats, Vegetable fats, Oils, Animal oils, Vegetable oils, Fatty acids, Methyl compounds, Esters (carboxylic), Chromatography, Gas chromatography, Column chromatography, Determination of content, Chemical analysis and testing, Test equipment, Testing conditions, Resolution, Error correction, Reproducibility
ICS67.200.10
Title in FrenchCorps gras d'origines animale et végétale. Chromatographie en phase gazeuse des esters méthyliques d'acides gras. Lignes directrices relatives à la chromatographie en phase gazeuse moderne des esters méthyliques d'acides gras
Title in GermanTierische und pflanzliche Fette und Öle. Gaschromatographie von Fettsäuremethylestern. Leitfaden für die moderne Gaschromatographie von Fettsäuremethylestern
CommitteeAW/307
ISBN978 0 580 90475 2
PublisherBSI
FormatA4
DeliveryYes
Pages18
File Size1.031 MB
Price£130.00


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