ASTM F3088 - 14 - Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

ASTM F3088 - 14

Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

Status : Current   Published : January 2014

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1.1 This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.

1.2 This test method does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.

1.3 The cells may include adult, progenitor or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.

1.4 This test method does not cover methods for isolating or harvesting of cells. This test method does not cover test methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this test method cover quantitative image analysis techniques.

1.5  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.




Standard NumberASTM F3088 - 14
TitleStandard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions
StatusCurrent
Publication Date05 January 2014
Normative References(Required to achieve compliance to this standard)No other standards are normatively referenced
Informative References(Provided for Information)No other standards are informatively referenced
Descriptors Cell adhesion assay, Centrifugation, Chondrocytes, Monolayer culture, Serial passage, Tissue culture-treated polystyrene
ICS07.080
PublisherASTM
FormatA4
DeliveryYes
Pages7
File Size0 KB
Price£40.00


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