PD CEN/TS 16707:2014 - Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies

PD CEN/TS 16707:2014

Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies

Status : Current, Under review   Published : October 2014

Format
PDF

Format
HARDCOPY



This Technical Specification describes screening strategies for the detection of genetically modified (GM) DNA in food products by means of PCR methods. The strategies have been established for food matrices, but it can also be applied to other matrices (e.g. feed, seed and samples from field grown plants).

Detection of GM DNA is based on PCR methods targeting segments of transgenic DNA sequences (genetic elements, genetic constructs or insertion sites of transgenes). Various combinations of these PCR methods are involved in screening strategies. The methods are applied simultaneously or hierarchically. The general strategy is based on the matrix approach. Examples for the implementation and application of this approach are described.

In order to ensure reliable analytical results, the document also provides guidelines for the validation of the performance of qualitative PCR methods applied in screening approaches.




Standard NumberPD CEN/TS 16707:2014
TitleFoodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies
StatusCurrent, Under review
Publication Date31 October 2014
Confirm Date31 May 2017
Normative References(Required to achieve compliance to this standard)EN ISO 24276, EN ISO 21569, ISO 21569, ISO 21571, EN ISO 21570, ISO 24276, EN ISO 21571, ISO 21570
Informative References(Provided for Information)ISO 5725
DescriptorsContaminant determination (food), Test specimens, Reproducibility, Food testing, Qualitative analysis, Nucleic acids, Extraction methods of analysis, Food products, Biochemical methods of analysis, Chemical analysis and testing, Proteins, Genetically modified organisms, Sampling methods, Deoxyribonucleic acid, Quantitative analysis
ICS67.050
Title in FrenchProduits alimentaires. Méthodes d'analyse pour la détection des organismes génétiquement modifiés et des produits dérivés. Stratégies de criblage basées sur l'utilisation de la réaction de polymérisation en chaîne (PCR)
Title in GermanLebensmittel. Verfahren zum Nachweis von gentechnisch veränderten Organismen und ihren Produkten. Strategien für das Screening mit Polymerase-Kettenreaktion (PCR)
CommitteeAW/275
ISBN978 0 580 83732 6
PublisherBSI
FormatA4
DeliveryYes
Pages20
File Size1.149 MB
Price£130.00


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