PD CEN/TS 15634-2:2012 - Foodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR

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PD CEN/TS 15634-2:2012

Foodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR

Status : Superseded, Withdrawn   Published : February 2012 Replaced By : BS EN 15634-2:2019

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This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e.g. frankfurter, wiener). Real-time PCR detection of celery is based on a 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank acc. no. AF067082) of celery (Apium graveolens). The method has been validated on emulsion-type sausages (Bavarian "Leberkäse") spiked with celery. For this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with ground celery seeds or alternatively celery root powder, both at a 1000 mg/kg level. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 °C for 60 min [2].




Standard NumberPD CEN/TS 15634-2:2012
TitleFoodstuffs. Detection of food allergens by molecular biological methods. Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR
StatusSuperseded, Withdrawn
Publication Date29 February 2012
Withdrawn Date11 October 2019
Cross ReferencesEN 15634-1, EN ISO 24276, ISO 24246
Replaced ByBS EN 15634-2:2019
International RelationshipsCEN/TS 15634-2:2012
DescriptorsAllergies, Deoxyribonucleic acid, Food testing, Biochemical methods of analysis, Biochemistry, Food products, Qualitative analysis, Nucleic acids
ICS07.100.30
67.050
Title in FrenchProduits alimentaires. Détection des allergènes alimentaires par des méthodes d'analyse de biologie moléculaire. Céleri (Apium graveolens). Détermination qualitative d'une séquence d'ADN spécifique dans des saucisses cuites par PCR en temps réel
Title in GermanLebensmittel. Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren. Sellerie (Apium graveolens). Qualitative Bestimmung einer spezifischen DNA-Sequenz in Brühwürsten mittels Real-time-PCR
CommitteeAW/275
ISBN978 0 580 75517 0
PublisherBSI
FormatA4
DeliveryYes
Pages22
File Size1.112 MB
Price£126.00


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