BS EN 16204:2012 - Foodstuffs. Determination of lipophilic algal toxins (okadaic acid group toxins, yessotoxins, azaspiracids, pectenotoxins) in shellfish and shellfish products by LC-MS/MS

BS EN 16204:2012

Foodstuffs. Determination of lipophilic algal toxins (okadaic acid group toxins, yessotoxins, azaspiracids, pectenotoxins) in shellfish and shellfish products by LC-MS/MS

Status : Confirmed, Current   Published : May 2012

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This European Standard specifies a multi-reference method for the determination of lipophilic algal toxins (fat-soluble algal toxins produced by some dinoflagellates) in raw shellfish and shellfish products including cooked shellfish, by liquid chromatography coupled to tandem mass spectrometry LC-MS/MS [1], [2], [3]. This method has been validated in an inter-laboratory study consisting of three parts via the analysis of both naturally contaminated homogenates of blue mussel and spiked extracts of blue mussel, oyster and clam. For further information on the validation, see Annex A. Additional studies have investigated further matrices (see [4], [5]). The detection limit for toxins of the okadaic acid group, azaspiracids and pectenotoxins was determined to be 6 µg/kg shellfish meat and for yessotoxins 10 µg/kg shellfish meat. Quantitative determination of okadaic acid (OA), pectenotoxin 2 (PTX-2), azaspiracid-1 (AZA-1) and yessotoxin (YTX) can be carried out directly by means of standard substances available commercially. Assuming an equal response factor, okadaic acid is used for the indirect quantitative determination of the two dinophysistoxins dinophysistoxin-1 (DTX-1) and dinophysistoxin 2 (DTX-2); likewise azaspiracid 1 (AZA-1) is used for the indirect quantitative determination of azaspiracid-2 (AZA-2) and azaspiracid-3 (AZA-3), while YTX is used for homo-yessotoxin, 45-OH-yessotoxin and 45-OH-homo-yessotoxin, and PTX-2 for pectenotoxin-1 (PTX-1). The limit of quantification (LOQ) for toxins of the okadaic acid group, azaspiracids and pectenotoxins was determined to be 20 µg/kg shellfish meat and for yessotoxins 35 µg/kg shellfish meat. By means of hydrolysis [6], the esters of okadaic acid, DTX-1 and DTX-2 can also be determined quantitatively as the corresponding free acids.




Standard NumberBS EN 16204:2012
TitleFoodstuffs. Determination of lipophilic algal toxins (okadaic acid group toxins, yessotoxins, azaspiracids, pectenotoxins) in shellfish and shellfish products by LC-MS/MS
StatusConfirmed, Current
Publication Date31 May 2012
Confirm Date15 February 2018
Normative References(Required to achieve compliance to this standard)EN ISO 3696:1995
Informative References(Provided for Information)ISO 5725-4:1994, ISO 5725-6:1994, ISO 5725-2:1994, Commission Regulation 15/2011, Regulation (EC) No 2074/2005
International RelationshipsEN 16204:2012
Draft Superseded By10/30234561 DC
DescriptorsFood products, Quantitative analysis, Mussels, Toxins, Liquid chromatography, Food testing, Fish (meat), Chemical analysis and testing, Shellfish, Determination of content
ICS67.120.30
Title in FrenchProduits alimentaires. Dosage des toxines algales lipophiles (toxines du groupe acide okadaïque, yessotoxines, azaspiracides, pecténotoxines) dans les coquillages et les produits à base de coquillages par CLSM/ SM
Title in GermanLebensmittel. Bestimmung der lipophilen Algentoxine (Okadasäuregruppen-Toxine, Yessotoxine, Azaspirosäuren, Pectenotoxine) in Schalentieren und Schalentiererzeugnissen mit LC-MS/MS
CommitteeAW/275
ISBN978 0 580 72863 1
PublisherBSI
FormatA4
DeliveryYes
Pages44
File Size635 KB
Price£214.00


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