BS EN ISO 12966-3:2009 - Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters using trimethylsulfonium hydroxide – BSI British Standards

BS EN ISO 12966-3:2009

Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters using trimethylsulfonium hydroxide

Status : Revised, Withdrawn   Published : January 2010 Replaced By : BS EN ISO 12966-3:2016

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BS EN ISO 12966-3:2009 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters using trimethylsulfonium hydroxide

BS EN ISO 12966-3 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils including milk fat and blends containing milk fat. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit.

As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA). As CLA is not correctly analysed, this method is not applicable to the determination of the complete fatty acid composition of milk fat samples.

Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.

This part of BS EN ISO 12966 is based upon German Standard Method C-VI 11e (98).

Contents of BS EN ISO 12966-3:

1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of the test sample
8 Procedure
9 Test report


 




Standard NumberBS EN ISO 12966-3:2009
TitleAnimal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters. Preparation of methyl esters using trimethylsulfonium hydroxide
StatusRevised, Withdrawn
Publication Date31 January 2010
Withdrawn Date30 June 2016
Normative References(Required to achieve compliance to this standard)ISO 661
Informative References(Provided for Information)ISO 835, ISO 1042, ISO 5555
Replaced ByBS EN ISO 12966-3:2016
International RelationshipsEN ISO 12966-3:2009,ISO 12966-3:2009
Draft Superseded By08/30193158 DC
DescriptorsAnimal fats, Animal oils, Vegetable fats, Vegetable oils, Fats, Food products, Food testing, Chemical analysis and testing, Determination of content, Fatty acids, Esters (carboxylic), Methyl compounds, Esterification, Milk, Gas chromatography, Specimen preparation
ICS67.200.10
Title in FrenchCorps gras d'origines animale et végétale. Chromatographie en phase gazeuse des esters méthyliques d'acides gras. Préparation des esters méthyliques à l'aide d'hydroxyde de triméthylsulfonium (TMSH)
Title in GermanTierische und pflanzliche Fette und Öle. Gaschromatographie von Fettsäuremethylestern. Herstellung von Methylestern mittels Trimethylsulfoniumhydroxid (TMSH)
CommitteeAW/307
ISBN978 0 580 65361 2
PublisherBSI
FormatA4
DeliveryNo
Pages12
File Size362 KB
Price£110.00


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