BS EN 12821:2009 - Foodstuffs. Determination of vitamin D by high performance liquid chromatography. Measurement of cholecalciferol (D3) or ergocalciferol (D2)

BS EN 12821:2009

Foodstuffs. Determination of vitamin D by high performance liquid chromatography. Measurement of cholecalciferol (D3) or ergocalciferol (D2)

Status : Confirmed, Current   Published : April 2009

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HARDCOPY



This European Standard specifies a method for the determination of vitamin D3 (cholecalciferol) or vitamin D2 (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC). Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Both vitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable for samples with a content of vitamin D3 and vitamin D2. Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the corresponding metabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. This European Standard does only include measurement of vitamin D3 or vitamin D2. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst can define his own analytical work in accordance to the standard procedure. This method has been validated in inter-laboratory tests on fortified and non-fortified samples such as margarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4 ?g/100 g to 14 ?g/100 g. Further information on the validation data is given in Annex D.




Standard NumberBS EN 12821:2009
TitleFoodstuffs. Determination of vitamin D by high performance liquid chromatography. Measurement of cholecalciferol (D3) or ergocalciferol (D2)
StatusConfirmed, Current
Publication Date30 April 2009
Confirm Date01 February 2019
Normative References(Required to achieve compliance to this standard)ISO 3696:1987, EN ISO 3696
Informative References(Provided for Information)ISO 5725:1986
ReplacesBS EN 12821:2000
International RelationshipsEN 12821:2009
Draft Superseded By07/30158610 DC
DescriptorsDetermination of content, Vitamins, Extraction methods of analysis, Test equipment, Chemical analysis and testing, Specimen preparation, Food testing, Vitamin D, Samples, Liquid chromatography, Precision, Food products
ICS67.050
Title in FrenchProduits alimentaires. Dosage de la vitamine D par chromatographie liquide haute performance. Dosage du cholécalciférol (D3) et de l' ergocalciférol (D2)
Title in GermanLebensmittel. Bestimmung von Vitamin D mit Hochleistungs-Flüssigchromatographie. Bestimmung von Cholecalciferol (D3) oder Ergocalciferol (D2)
CommitteeAW/275
ISBN978 0 580 57954 7
PublisherBSI
FormatA4
DeliveryYes
Pages28
File Size527 KB
Price£182.00


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